Recombinant Anti-EPO-R (phospho Y454 + Y456) antibody [RP23040088] (ab308034) (2024)

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Recombinant Anti-EPO-R (phospho Y454 + Y456) antibody [RP23040088] (ab308034) (1)

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  • Form


  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99.91% PBS

  • Recombinant Anti-EPO-R (phospho Y454 + Y456) antibody [RP23040088] (ab308034) (2)Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Recombinant Multiclonal

  • Clone number


  • Isotype


  • Research areas

    • Immunology
    • Immunoglobulins
    • Receptors
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Other
    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Cardiovascular
    • Atherosclerosis
    • Ischemia / Reperfusion
    • Cardiovascular
    • Heart
    • Apoptosis
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers


The Abpromise guarantee

Our Abpromise guarantee covers the use of ab308034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes

1/500. Detects a band of approximately 28 kDa (predicted molecular weight: 55 kDa).




1/500. Detects a band of approximately 28 kDa (predicted molecular weight: 55 kDa).



  • Function

    Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.
    Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling.

  • Tissue specificity

    Erythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early-stage erythroid progenitor cells.

  • Involvement in disease

    Defects in EPOR are the cause of erythrocytosis familial type 1 (ECYT1) [MIM:133100]. ECYT1 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin and hematocrit, hypersensitivity of erythroid progenitors to erythropoietin, erythropoietin low serum levels, and no increase in platelets nor leukocytes. It has a relatively benign course and does not progress to leukemia.

  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 1 subfamily.
    Contains 1 fibronectin type-III domain.

  • Domain

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
    Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

  • Post-translational

    On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site.
    Ubiquitinated by NOSIP; appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells.

  • Cellular localization

    Cell membrane and Secreted. Secreted and located to the cell surface.

  • Information by UniProt
  • Database links

  • Alternative names

    • EPO R antibody
    • EPO Receptor antibody
    • EPO-R antibody

    see all


  • Western blot - Anti-EPO-R (phospho Y454 + Y456) antibody [RP23040088] (ab308034)

    All lanes : Anti-EPO-R (phospho Y454 + Y456) antibody [RP23040088] (ab308034) at 1/500 dilution

    Lane 1 : untreated HeLa cell lysate
    Lane 2 : untreated K562 cell lysate
    Lane 3 : phosphopeptide incubated HeLa cell lysate
    Lane 4 : phosphopeptide incubated K562 cell lysate

    Lysates/proteins at 30 µg per lane.

    All lanes : Goat anti-Rabbit IgG - HRP Secondary Antibody at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 55 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?

    Samples were loaded using a 4-12% Bis-Tris gel, electrophoresis system, and pre-stained protein standard.

  • Immunocytochemistry/ Immunofluorescence - Anti-EPO-R (phospho Y454 + Y456) antibody [RP23040088] (ab308034)

    For immunofluorescence analysis, 70% confluent log phase HeLa cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature for detection of EpoR (phospho T454 + T456) using Ab308034 at 1/500 dilution in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Goat anti-Rabbit IgG Secondary Antibody at a dilution of 1/400 for 30 minutes at room temperature (Panel a: green)

    F-actin (Panel b: red) was stained with Alexa Fluor® 594 Phalloidin

    Nuclei (Panel c: blue) were stained with DAPI

    Panel d is a merged image showing cytoplasmic and membrane localization

    Panel e shows competition with the EpoR (phospho T454 + T456)peptide

    The images were captured at 20X magnification

  • Anti-EPO-R (phospho Y454 + Y456) antibody [RP23040088] (ab308034)


To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

    Recombinant Anti-EPO-R (phospho Y454 + Y456) antibody [RP23040088] (ab308034) (6)

  • Datasheet download


References (0)

Publishing research using ab308034? Please let us know so that we can cite the reference in this datasheet.

ab308034 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Recombinant Anti-EPO-R (phospho Y454 + Y456) antibody [RP23040088] (ab308034) (7)

Recombinant Anti-EPO-R (phospho Y454 + Y456) antibody [RP23040088] (ab308034) (2024)
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